The composition of the scientific group: Head of the Project – Doctor of Biology, Assistant Professor Yuryi Prysedskyi; Principal investigator – senior lecturer Kateryna Reshetnyk; Investigators: Assistant Alina Kutsevol, Assistant Ruslan Kudlayev, Laboratory assistant Dmytro Yuskov, Laboratory assistant Viktoriya Klochanyuk
The research team was established in 2014. The staff is studying the effect of LED laser systems on the growth of basidiomycetes, agricultural and medicinal plants in order to increase their productivity and improve product quality. The mechanisms helping to stimulate the growth of plants and fungi are studied.
Short description of the project
The project aims at developing the ways to increase yields and adaptive capacity of agricultural plants through pre-treatment of seeds using LED laser systems, introducing methods for intensifying growth of edible basidiomycetes and xylotrophs – producers of biologically active substances using laser irradiation of mycelium.
Tasks to solve:
- study how combined monochromatic irradiation using LED laser systems (635 nm, 532 nm and 405 nm) influences the initial growth of some agricultural, medicinal and ornamental plants;
- study how seed irradiation influences the meristematic activity of apical cells in experimental plants;
- study the dynamics of growth and productivity of plants grown from irradiated seeds;
- determine some biochemical parameters of plants grown from seeds irradiated with LED laser systems;
- establish optimal conditions for irradiation of different seeds by building statistical models;
- find ways to practically apply the obtained results and increase the productivity of agricultural and medicinal plants;
- study how different spectra of laser radiation influence the growth rate, biomass accumulation and fruiting bodies of industrial strains of edible mushrooms;
- investigate the possibility of increasing the biosynthetic activity of xylotrophic basidiomycetes – producers of enzymes of antioxidant nature by laser irradiation;
- develop methods for intensifying the accumulation of biomass and biosynthetic activity of xylotrophic macromycetes – producers of carotenoids;
- determine the possibility of increasing the adaptive properties of medicinal macromycetes under the influence of laser irradiation.
It was found that irradiation by red (635 nm) and blue (45 nm) laser probably increases the germination energy of seeds of cereals, oil plants and vegetables by 0.6–51.2% compared to plants grown from non-irradiated seeds. Irradiation positively effected the content of chlorophyll a and chlorophyll b in all the cereals under study. Also, the height of aboveground portions of plants and the root growth significantly increased. Field studies showed that pre-sowing treatment of corn seeds by red and blue coherent monochromatic light was highly effective. Irradiating the seeds with blue light for 5 seconds increased the mass of corncobs by 148.2%, while complex irradiation (var. 6 and 8) led to an increase in the mass of corncobs by 372-44.4%.
The studies show that the most effective way to stimulate the growth of oyster mushrooms is to irradiate the mycelium with a green LED laser for 10 sec. This increases mycelial growth rate by 38.3%. The number of primordia increase significantly, which led to an increase in the yield of fruiting bodies of oyster mushroom.
The results of experimental research expand the fundamental knowledge about the mechanisms of photoactivation of physiological processes in plants and fungi, which can be used in agriculture, mycology and biotechnology. After practical implementation we will develop complex methods of photostimulation of seeds of agricultural plants to be introduced in production; as well as methods of irradiation of vegetative mycelium for optimized cultivation of strains of basidiomycetes – producers of oxidoreductase enzymes and carotenoids; and methods of growth stimulation in new highly productive cultures of edible mushrooms.
Among the equipment required for project implementation there is a device for monochromatic light irradiation using LED lasers, which was developed by the staff of the Department of Botany and Ecology – Acting Head Yurii Prysedsky, senior lecturer Kateryna Reshetnyk and laboratory assistant Dmytro Yuskov (Fig. 1). Also, the implementation of the project requires an autoclave, thermostat, refrigerator, pH meter, spectrophotometer, which are available.
Figure 1. device for monochromatic light irradiation using LED lasers
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