Faculty of Chemistry, Biology and Biotechnology, Department of Botany and Ecology
Finding ways to increase the productivity of plants and mushrooms using led laser systems

The composition of the scientific group: Head of the Project – Doctor of Biology, Assistant Professor Yuryi Prysedskyi; Principal investigator – senior lecturer Kateryna Reshetnyk; Investigators: Assistant Alina Kutsevol, Assistant Ruslan Kudlayev, Laboratory assistant Dmytro Yuskov, Laboratory assistant Viktoriya Klochanyuk

General information about the research group implementing the project

The research team was established in 2014. The staff is studying the effect of LED laser systems on the growth of basidiomycetes, agricultural and medicinal plants in order to increase their productivity and improve product quality. The mechanisms helping to stimulate the growth of plants and fungi are studied.

Short description of the project

The project aims at developing the ways to increase yields and adaptive capacity of agricultural plants through pre-treatment of seeds using LED laser systems, introducing methods for intensifying growth of edible basidiomycetes and xylotrophs – producers of biologically active substances using laser irradiation of mycelium.

Tasks to solve:

  • study how combined monochromatic irradiation using LED laser systems (635 nm, 532 nm and 405 nm) influences the initial growth of some agricultural, medicinal and ornamental plants;
  • study how seed irradiation influences the meristematic activity of apical cells in experimental plants;
  • study the dynamics of growth and productivity of plants grown from irradiated seeds;
  • determine some biochemical parameters of plants grown from seeds irradiated with LED laser systems;
  • establish optimal conditions for irradiation of different seeds by building statistical models;
  • find ways to practically apply the obtained results and increase the productivity of agricultural and medicinal plants;
  • study how different spectra of laser radiation influence the growth rate, biomass accumulation and fruiting bodies of industrial strains of edible mushrooms;
  • investigate the possibility of increasing the biosynthetic activity of xylotrophic basidiomycetes – producers of enzymes of antioxidant nature by laser irradiation;
  • develop methods for intensifying the accumulation of biomass and biosynthetic activity of xylotrophic macromycetes – producers of carotenoids;
  • determine the possibility of increasing the adaptive properties of medicinal macromycetes under the influence of laser irradiation.

The most significant results achieved

It was found that irradiation by red (635 nm) and blue (45 nm) laser probably increases the germination energy of seeds of cereals, oil plants and vegetables by 0.6–51.2% compared to plants grown from non-irradiated seeds. Irradiation positively effected the content of chlorophyll a and chlorophyll b in all the cereals under study. Also, the height of aboveground portions of plants and the root growth significantly increased. Field studies showed that pre-sowing treatment of corn seeds by red and blue coherent monochromatic light was highly effective. Irradiating the seeds with blue light for 5 seconds increased the mass of corncobs by 148.2%, while complex irradiation (var. 6 and 8) led to an increase in the mass of corncobs by 372-44.4%.

The studies show that the most effective way to stimulate the growth of oyster mushrooms is to irradiate the mycelium with a green LED laser for 10 sec. This increases mycelial growth rate by 38.3%. The number of primordia increase significantly, which led to an increase in the yield of fruiting bodies of oyster mushroom.

Application areas

The results of experimental research expand the fundamental knowledge about the mechanisms of photoactivation of physiological processes in plants and fungi, which can be used in agriculture, mycology and biotechnology. After practical implementation we will develop complex methods of photostimulation of seeds of agricultural plants to be introduced in production; as well as methods of irradiation of vegetative mycelium for optimized cultivation of strains of basidiomycetes – producers of oxidoreductase enzymes and carotenoids; and methods of growth stimulation in new highly productive cultures of edible mushrooms.

Available equipment

Among the equipment required for project implementation there is a device for monochromatic light irradiation using LED lasers, which was developed by the staff of the Department of Botany and Ecology – Acting Head Yurii Prysedsky, senior lecturer Kateryna Reshetnyk and laboratory assistant Dmytro Yuskov (Fig. 1). Also, the implementation of the project requires an autoclave, thermostat, refrigerator, pH meter, spectrophotometer, which are available.

Figure 1. device for monochromatic light irradiation using LED lasers

Important publications

  1. Reshetnyk, K., Prysedskyi, Yu. Method of growth stimulation of dikaryotic mycelium of edible fungus (Pleurotus ostreatus (Jacq.) P. Kumm): Pat. 124287 Ukraine. No. u 201712431, dated 14.12.2017, IPC (2018.01), Bull.No. 6, dated March 26, 2018 (in Ukrainian).
  2. Reshetnyk, K., Prysedskyi, Yu. Declarative utility model patent of Ukraine No.124286. The method of stimulating the growth of the medicinal fungus Schizophyllum Commune Application No. u 201712427, dated 14.12.2017, IPC (2018.01), Bull. No. 6, dated March 26, 2018 (in Ukrainian).
  3. Reshetnyk, K., Prysedskyi, Yu. Declarative utility model patent of Ukraine No.124612. The method of stimulating the initial growth of the medicinal fungus Schizophyllum Commune Application No. u 201712432, dated 14.12.2017, IPC (2017.01), Bull.No. 7, dated April 10, 2018 (in Ukrainian).
  4. Reshetnyk, K., Prysedskyi, Yu. Declarative utility model patent of Ukraine No.124610. A method of stimulating the growth of the edible fungus (Pleurotus ostreatus (Jacq.) P. Kumm). Application No. u 201712425, dated 14.12.2017, IPC (2018.01), Bull. No. 7, dated April 10, 2018 (in Ukrainian).
  5. Prysedskyi Yu., Lagunova, A., Gutianska, S. The method of accelerating the initial growth of agricultural plants: Pat. No. 114566, No.u201609844; publ. 10.03.2017, Bull.No. 5 (in Ukrainian).
  6. Prysedskyi Yu., Lagunova, A., Gutianska, S. The method of stimulating initial growth of roots in agricultural plants: Pat. 114882 Ukraine, No.u201609821; publ. 27.03.2017, Bull.No. 6 (in Ukrainian).
  7. Reshetnyk, K., Prysedskyi, Yu., Yuskov, D. Declarative utility model patent of Ukraine No. 877419. Method of intensifying the growth of higher basidiomycete fungus Flammulina velutipes. Application No. u201811990, dated 03.12.2018, IPC A01G 18/40 (2018.01), Bull.No. 7, dated April 9, 2019 (in Ukrainian).
  8. Reshetnyk, K., Yuskov, D. Declarative utility model patent of Ukraine No. 996219. Method of increasing the activity of the antioxidant system of Pleurotus ostreatus Application No. u201812553, dated 17.12.2018, IPC A01G 18/40 (2018.01), Bull.No. 7, dated April 9, 2019 (in Ukrainian).

Articles in journals indexed by Web of Science

  1. Reshetnyk K., Prysedsky Yu., Yuskov D. The influence of laser irradiation on the development of vegetative micelium Pleurotus ostreatus. 2019. Vol.65, No.4. Р. 243–250.

Articles in foreign journals included in other international

Scientometric databases

  1. Reshetnyk K., Yuskov D. Modification of peroxidase activity of some stains of basidiomycota under the influence of laser radiation. The scientific heritage. Biological Sciences. 2019. Vol.39, No 1. С. 12–14.
  2. Reshetnyk K., Yuskov D., Levytska D., Senyk N., Some xylotrophic mushrooms of the Podil’s region. The scientific heritage. Biological Sciences. 2019. Vol.40, No 2. С. 17–20.

Articles in scientific journals of Ukraine

  1. Reshetnyk, K., Yuskov, D. Catalase activity of the macromycete Pleurotus ostreatus (Jacq. Fr) P. Kumm under the action of laser irradiation. ScienceRise: Biological Science. Vol. 1, No 16. P. 30–36 (in Ukrainian).
  2. Reshetnyk, K. Cultivation of Pleurotus ostreatus (Jacq.: Fr.) Kumm. under the action of laser irradiation. Bulletin of Kharkiv National University. Biology series. 2019. No. 33 (2). P. 71–83 (in Ukrainian).
  3. Reshetnyk, K., Yuskov, D. Influence of laser irradiation on the catalase activity of basidiomycete Flammulina velutipes (Curt.: Fr.) Sing. Bulletin of Lviv University. Biology series. 2019. No. 81. P. 3-11 (in Ukrainian).
  4. Reshetnyk, K. Influence of LED lasers on growth processes of macromycete Flammulina velutipes (Curt.: Fr.) Sing. Scientific reports of NUBiP of Ukraine. Biology series. 2019. No. 81(5). P. 1-8 (in Ukrainian).
  5. Hutianska, S. Prysedskyi Yu. Influence of laser irradiation of seeds on growth processes and pigment content in oilseeds. Scientific reports of NUBiP of Ukraine. 2017. No. 2 (66) (in Ukrainian).
  6. Nishenko, L., Prysedskyi, Yu. The effect of laser pretreatment of seeds on robinia’s growth and chlorophyll content in it under conditions when soil is contaminated with sulfur and fluorine compounds. Scientific reports of NUBiP of Ukraine. Biology series. 2017. No. 1 (65) (in Ukrainian).
Contacts:

Head of the Project – Doctor of Biology, Assistant Professor Prysedskyi Yu.

Tel. +380977503811; email: yu.prysedskyi@donnu.edu.ua

Principal investigator – senior lecturer Reshetnyk K.

Tel. +380969515529; email: k.reshetnyk@donnu.edu.ua